Objective to examine the metabolic change pathways of 4F-MDMB-BUTINACA in vivo by establishing zebrafish designs. Practices Six adult zebrafish were randomly divided into blank control team and experimental group, with three fish in each team. After the zebrafish in the experimental group were subjected to 1 μg/mL 4F-MDMB-BUTINACA for 24 h, these people were used in clean water and cleaned 3 x, then pretreated for instrumental evaluation. The zebrafish in empty control group are not exposed to 4F-MDMB-BUTINACA. Mass spectrometry and structural evaluation of 4F-MDMB-BUTINACA and its metabolites had been carried out by liquid chromatography-high resolution size spectrometry and Mass Frontier pc software. Outcomes an overall total of twenty-six metabolites of 4F-MDMB-BUTINACA were identified in zebrafish, including eighteen period Ⅰ metabolites and eight phase Ⅱ metabolites. The main metabolic pathways of period Ⅰ metabolites of 4F-MDMB-BUTINACA in zebrafish had been ester hydrolysis, N-dealkylation, oxidative defluorination anmetabolites of 4F-MDMB-BUTINACA were identified in zebrafish, including eighteen phase Ⅰ metabolites and eight phase Ⅱ metabolites. The primary metabolic pathways of period Ⅰ metabolites of 4F-MDMB-BUTINACA in zebrafish had been ester hydrolysis, N-dealkylation, oxidative defluorination and hydroxylation, as the Medical utilization main metabolic pathway of phase Ⅱ metabolites was glucuronidation. Conclusion Metabolite Md24 （ester hydrolysis） and Md25 （ester hydrolysis combined with dehydrogenation） would be advised becoming potentially good biomarkers for punishment of 4F-MDMB-BUTINACA. Objective to review the qualitative evaluation strategy for unknown synthetic cannabinoid when you look at the dubious herbal item when no reference substance can be acquired. Techniques The synthetic cannabinoid in natural blend was removed with methanol. The herb was concentrated by rotary evaporator and isolated and purified by preparative liquid chromatography, to acquire high purity synthetic cannabinoid sample. Gasoline chromatography-mass spectrometry （GC-MS）, ultra-high overall performance liquid chromatography-quadrupole time-of-flight mass spectrometry （UPLC-QTOF-MS） and nuclear magnetic resonance （NMR） were utilized to determine the framework associated with the prepared chemical. Results High purity unknown sample （10 mg） had been acquired by preparative fluid chromatography. The sample ended up being reviewed by GC-MS, UPLC-TOF-MS and NMR, and through range evaluation genetic test , the unknown synthetic cannabinoid ended up being determined as 5F-EDMB-PICA. Conclusion The method to extract unknown synthetic cannabinoid from low material organic products by preparative liquid tography. The test had been examined by GC-MS, UPLC-TOF-MS and NMR, and through range evaluation, the unknown artificial cannabinoid was determined as 5F-EDMB-PICA. Conclusion the strategy to draw out unknown artificial cannabinoid from reduced material herbal products by preparative fluid chromatography ended up being set up, additionally the framework regarding the unknown test was identified by comprehensive utilization of GC-MS, UPLC-QTOF-MS and NMR. The information and knowledge can assist forensic laboratories in identifying this compound or other substances with similar structures within their casework. Goal To establish a recognition means for common brand new psychoactive substances of synthetic cannabinoids in tresses with ultra-high overall performance fluid chromatography-tandem mass spectrometry （UPLC-MS/MS）. Techniques In the 1 mL of internal standard methanol answer, 20 mg hair had been added. After cryogenic grinding and ultrasonic removal, the herb ended up being separated by ACQUITY UPLC HSS T >0.99）, the limitations of recognition had been 0.5-2 pg/mg, the limitations of quantification were 1-5 pg/mg, the intra-day and inter-day precisions were 0.1%-12.6%, the intra-day and inter-day accuracies had been 89.2%-110.7%, the recovery prices were 52.3%-93.3%, while the matrix results were 19.1%-95.2%. Conclusion The established method has an easy sample planning procedure and high susceptibility. It really is appropriate qualitative and quantitative analysis of typical brand new psychoactive substances of synthetic cannabinoids in tresses.0.99）, the limitations of recognition had been 0.5-2 pg/mg, the limits of measurement were 1-5 pg/mg, the intra-day and inter-day precisions were 0.1%-12.6%, the intra-day and inter-day accuracies had been 89.2%-110.7%, the recovery rates were 52.3%-93.3%, plus the matrix impacts had been 19.1%-95.2%. Conclusion The set up strategy features a simple sample preparation procedure and large susceptibility. Its suitable for qualitative and quantitative evaluation of common brand new psychoactive substances of artificial cannabinoids in hair. In the past few years, due to the fact third-generation of medicines, new psychoactive substances （NPS） have actually expanded quickly and be a significant issue for China’s anti-drug prevention and control system. As a fresh drug monitoring technology in today’s anti-drug industry, wastewater evaluation is a target, real-time, accurate, convenient and efficient medication tracking method. In the past few years, it has slowly been placed on the tabs on NPS. This study summarizes wastewater test collection, target stability analysis, wastewater test pretreatment, wastewater test evaluation methods, target NPS usage computations and real tracking applications, with a view to your building of a tracking system for NPS in wastewater, real time and accurate understanding of information from the usage of NPS in towns, the expression associated with the real use of several types of NPS and consumption styles in a short span of time, and forecast associated with the development trend of abused usage, which is of good value for combatinf time, and prediction associated with development trend of abused use, which is of great importance for fighting NPS crimes, serving and guaranteeing the personal Inobrodib protection of the people, and keeping personal stability.